Enable Two-Factor Authentication Enforcement
Two-Factor Authentication Enforcement can be enabled in your organization’s Members settings screen:

Grace periods
When Two-Factor Authentication Enforcement in enabled organisation members are given a grace period to set-up Two-Factor Authentication on their AppSignal accounts:
If a user does not enable Two-Factor Authentication before the end of their grace period they will locked out of the organization until it’s enabled:

Disable Two-Factor Authentication Enforcement
Two-Factor Authentication Enforcement can be disabled in your organization’s Members settings screen: